<?xml version="1.0" encoding="UTF-8"?>

<!--XBRL Document Created with Wdesk from Workiva-->
<!--Copyright 2022 Workiva-->
<!--r:78ec9333-7032-4de9-b77b-1e7012707322,g:61e2d6d7-c5e7-4f98-8dfa-0efe4c74d2f1-->
<link:linkbase xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:xbrldt="http://xbrl.org/2005/xbrldt" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd">
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/all" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#all"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#hypercube-dimension"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-default"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-domain"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/domain-member" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#domain-member"/>
  <link:roleRef roleURI="http://www.tempesttx.com/role/CondensedConsolidatedStatementsofStockholdersEquityDeficit" xlink:type="simple" xlink:href="tpst-20220930.xsd#CondensedConsolidatedStatementsofStockholdersEquityDeficit"/>
  <link:definitionLink xlink:role="http://www.tempesttx.com/role/CondensedConsolidatedStatementsofStockholdersEquityDeficit" xlink:type="extended" id="i24913f720b4043cda767dc392adaf393_CondensedConsolidatedStatementsofStockholdersEquityDeficit">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_d230690e-c6ee-484a-a567-f07ff8404b4b" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_6b536f27-d6dc-47ef-9da2-88aafd6cf150" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncreaseDecreaseInStockholdersEquityRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_d230690e-c6ee-484a-a567-f07ff8404b4b" xlink:to="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_6b536f27-d6dc-47ef-9da2-88aafd6cf150" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharesOutstanding_82d65aaa-29fa-40a9-9de7-5d54eb821fcb" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_SharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_6b536f27-d6dc-47ef-9da2-88aafd6cf150" xlink:to="loc_us-gaap_SharesOutstanding_82d65aaa-29fa-40a9-9de7-5d54eb821fcb" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_d3d9dbf9-abcc-4ebf-a307-a9f8bc8954ef" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StockholdersEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_6b536f27-d6dc-47ef-9da2-88aafd6cf150" xlink:to="loc_us-gaap_StockholdersEquity_d3d9dbf9-abcc-4ebf-a307-a9f8bc8954ef" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossForeignCurrencyTransactionAndTranslationAdjustmentNetOfTax_a6301d60-7131-40c2-9ac3-935971b2f03b" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OtherComprehensiveIncomeLossForeignCurrencyTransactionAndTranslationAdjustmentNetOfTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_6b536f27-d6dc-47ef-9da2-88aafd6cf150" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossForeignCurrencyTransactionAndTranslationAdjustmentNetOfTax_a6301d60-7131-40c2-9ac3-935971b2f03b" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesNewIssues_0302080d-9e95-464e-92e4-a09e8939786c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StockIssuedDuringPeriodSharesNewIssues"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_6b536f27-d6dc-47ef-9da2-88aafd6cf150" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesNewIssues_0302080d-9e95-464e-92e4-a09e8939786c" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueNewIssues_9b7a8c92-f0cc-47de-89a4-985e5038becd" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StockIssuedDuringPeriodValueNewIssues"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_6b536f27-d6dc-47ef-9da2-88aafd6cf150" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueNewIssues_9b7a8c92-f0cc-47de-89a4-985e5038becd" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised_5451dd27-0175-4fbb-b6de-df0e810a75da" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_6b536f27-d6dc-47ef-9da2-88aafd6cf150" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised_5451dd27-0175-4fbb-b6de-df0e810a75da" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueStockOptionsExercised_09378ac2-4e83-4980-a034-ffe9e884aa59" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StockIssuedDuringPeriodValueStockOptionsExercised"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_6b536f27-d6dc-47ef-9da2-88aafd6cf150" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueStockOptionsExercised_09378ac2-4e83-4980-a034-ffe9e884aa59" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_tpst_VestingOfEarlyExercisedStockOptionsAndRestrictedStockShares_9d2ce0fd-bb7a-4898-b197-ac90f6a2b298" xlink:href="tpst-20220930.xsd#tpst_VestingOfEarlyExercisedStockOptionsAndRestrictedStockShares"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_6b536f27-d6dc-47ef-9da2-88aafd6cf150" xlink:to="loc_tpst_VestingOfEarlyExercisedStockOptionsAndRestrictedStockShares_9d2ce0fd-bb7a-4898-b197-ac90f6a2b298" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_tpst_VestingOfEarlyExercisedStockOptionsAndRestrictedStock_a97d5fe1-7193-44e0-9921-b15a686e8a92" xlink:href="tpst-20220930.xsd#tpst_VestingOfEarlyExercisedStockOptionsAndRestrictedStock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_6b536f27-d6dc-47ef-9da2-88aafd6cf150" xlink:to="loc_tpst_VestingOfEarlyExercisedStockOptionsAndRestrictedStock_a97d5fe1-7193-44e0-9921-b15a686e8a92" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesConversionOfConvertibleSecurities_b851acdd-281a-483b-a72e-9e70d68c5cbc" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StockIssuedDuringPeriodSharesConversionOfConvertibleSecurities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_6b536f27-d6dc-47ef-9da2-88aafd6cf150" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesConversionOfConvertibleSecurities_b851acdd-281a-483b-a72e-9e70d68c5cbc" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueConversionOfUnits_5394b114-b860-4eaa-8775-89d4b0c87650" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StockIssuedDuringPeriodValueConversionOfUnits"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_6b536f27-d6dc-47ef-9da2-88aafd6cf150" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueConversionOfUnits_5394b114-b860-4eaa-8775-89d4b0c87650" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdjustmentsToAdditionalPaidInCapitalShareBasedCompensationStockOptionsRequisiteServicePeriodRecognition_1d747b9a-747d-4aa4-b22d-d1b9021fd7a9" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AdjustmentsToAdditionalPaidInCapitalShareBasedCompensationStockOptionsRequisiteServicePeriodRecognition"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_6b536f27-d6dc-47ef-9da2-88aafd6cf150" xlink:to="loc_us-gaap_AdjustmentsToAdditionalPaidInCapitalShareBasedCompensationStockOptionsRequisiteServicePeriodRecognition_1d747b9a-747d-4aa4-b22d-d1b9021fd7a9" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_tpst_AdjustmentsToAdditionalPaidInCapitalReverseRecapitalizationTransactionCosts_d5b4895e-d4d2-47e5-92bd-b5bb30eae85f" xlink:href="tpst-20220930.xsd#tpst_AdjustmentsToAdditionalPaidInCapitalReverseRecapitalizationTransactionCosts"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_6b536f27-d6dc-47ef-9da2-88aafd6cf150" xlink:to="loc_tpst_AdjustmentsToAdditionalPaidInCapitalReverseRecapitalizationTransactionCosts_d5b4895e-d4d2-47e5-92bd-b5bb30eae85f" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesOther_d96815e4-e57e-42b9-9dc9-449767e1af22" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StockIssuedDuringPeriodSharesOther"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_6b536f27-d6dc-47ef-9da2-88aafd6cf150" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesOther_d96815e4-e57e-42b9-9dc9-449767e1af22" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueOther_2818a1ce-725d-409b-b417-86177f277ee8" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StockIssuedDuringPeriodValueOther"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_6b536f27-d6dc-47ef-9da2-88aafd6cf150" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueOther_2818a1ce-725d-409b-b417-86177f277ee8" xlink:type="arc" order="15"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdjustmentsToAdditionalPaidInCapitalWarrantIssued_50cc235a-0dc1-4561-a9da-a32295264717" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AdjustmentsToAdditionalPaidInCapitalWarrantIssued"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_6b536f27-d6dc-47ef-9da2-88aafd6cf150" xlink:to="loc_us-gaap_AdjustmentsToAdditionalPaidInCapitalWarrantIssued_50cc235a-0dc1-4561-a9da-a32295264717" xlink:type="arc" order="16"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_e3c647c1-90d5-4583-a4f0-7283840d6194" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_6b536f27-d6dc-47ef-9da2-88aafd6cf150" xlink:to="loc_us-gaap_NetIncomeLoss_e3c647c1-90d5-4583-a4f0-7283840d6194" xlink:type="arc" order="17"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_9966eee9-d387-4c2d-95d2-024e0dbe31e4" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StockholdersEquity"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharesOutstanding_4065cb6c-a2aa-4c0c-89d4-6d42c8934682" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_SharesOutstanding"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_0c63117a-78fd-41fc-b492-93b033e367a0" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_d230690e-c6ee-484a-a567-f07ff8404b4b" xlink:to="loc_us-gaap_StatementTable_0c63117a-78fd-41fc-b492-93b033e367a0" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_29d62a7c-3cbb-4e72-8851-4c3f3f29b3f8" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StatementEquityComponentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_0c63117a-78fd-41fc-b492-93b033e367a0" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_29d62a7c-3cbb-4e72-8851-4c3f3f29b3f8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_29d62a7c-3cbb-4e72-8851-4c3f3f29b3f8_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_29d62a7c-3cbb-4e72-8851-4c3f3f29b3f8" xlink:to="loc_us-gaap_EquityComponentDomain_29d62a7c-3cbb-4e72-8851-4c3f3f29b3f8_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_e12b6348-11d5-4e55-9041-e636bd877b98" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_29d62a7c-3cbb-4e72-8851-4c3f3f29b3f8" xlink:to="loc_us-gaap_EquityComponentDomain_e12b6348-11d5-4e55-9041-e636bd877b98" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockMember_f632e8bc-13de-4bd3-a6c7-a2d6630e4bc7" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CommonStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_e12b6348-11d5-4e55-9041-e636bd877b98" xlink:to="loc_us-gaap_CommonStockMember_f632e8bc-13de-4bd3-a6c7-a2d6630e4bc7" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdditionalPaidInCapitalMember_28169795-a7a7-4e81-be4d-b3e62f6e38a0" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AdditionalPaidInCapitalMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_e12b6348-11d5-4e55-9041-e636bd877b98" xlink:to="loc_us-gaap_AdditionalPaidInCapitalMember_28169795-a7a7-4e81-be4d-b3e62f6e38a0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetainedEarningsMember_ef026480-139a-4aa5-86ee-f84ddf71aab0" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_RetainedEarningsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_e12b6348-11d5-4e55-9041-e636bd877b98" xlink:to="loc_us-gaap_RetainedEarningsMember_ef026480-139a-4aa5-86ee-f84ddf71aab0" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_3106b609-1801-4c27-b472-c1c5b88eb9e2" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_e12b6348-11d5-4e55-9041-e636bd877b98" xlink:to="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_3106b609-1801-4c27-b472-c1c5b88eb9e2" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.tempesttx.com/role/StockCompensationTables" xlink:type="simple" xlink:href="tpst-20220930.xsd#StockCompensationTables"/>
  <link:definitionLink xlink:role="http://www.tempesttx.com/role/StockCompensationTables" xlink:type="extended" id="i641024b8d07a487b8ab56bf1365a251f_StockCompensationTables">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_a16dbdbc-f510-43bf-9507-9f76ac53c55f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfCompensationCostForShareBasedPaymentArrangementsAllocationOfShareBasedCompensationCostsByPlanTableTextBlock_d608b908-c111-4124-aa22-a78f9ad1669d" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ScheduleOfCompensationCostForShareBasedPaymentArrangementsAllocationOfShareBasedCompensationCostsByPlanTableTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_a16dbdbc-f510-43bf-9507-9f76ac53c55f" xlink:to="loc_us-gaap_ScheduleOfCompensationCostForShareBasedPaymentArrangementsAllocationOfShareBasedCompensationCostsByPlanTableTextBlock_d608b908-c111-4124-aa22-a78f9ad1669d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShareBasedPaymentAwardStockOptionsValuationAssumptionsTableTextBlock_7a4a9821-d075-4de5-a61b-b3d013eec2b9" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ScheduleOfShareBasedPaymentAwardStockOptionsValuationAssumptionsTableTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_a16dbdbc-f510-43bf-9507-9f76ac53c55f" xlink:to="loc_us-gaap_ScheduleOfShareBasedPaymentAwardStockOptionsValuationAssumptionsTableTextBlock_7a4a9821-d075-4de5-a61b-b3d013eec2b9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsVestedAndExpectedToVestOutstandingTableTextBlock_edd0b9df-ee5c-42b7-bf6d-0ffc44cc7ebd" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ScheduleOfShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsVestedAndExpectedToVestOutstandingTableTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_a16dbdbc-f510-43bf-9507-9f76ac53c55f" xlink:to="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsVestedAndExpectedToVestOutstandingTableTextBlock_edd0b9df-ee5c-42b7-bf6d-0ffc44cc7ebd" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShareBasedCompensationStockOptionsActivityTableTextBlock_6b2572ac-f634-4e6b-90ac-2d94131fd495" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ScheduleOfShareBasedCompensationStockOptionsActivityTableTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_a16dbdbc-f510-43bf-9507-9f76ac53c55f" xlink:to="loc_us-gaap_ScheduleOfShareBasedCompensationStockOptionsActivityTableTextBlock_6b2572ac-f634-4e6b-90ac-2d94131fd495" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_22a91205-961e-4c24-a889-b3a0d8b5f7e4" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_a16dbdbc-f510-43bf-9507-9f76ac53c55f" xlink:to="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_22a91205-961e-4c24-a889-b3a0d8b5f7e4" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GranteeStatusAxis_6bad971a-33cb-4fe7-8cbb-afa1cfc4a48d" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_GranteeStatusAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_22a91205-961e-4c24-a889-b3a0d8b5f7e4" xlink:to="loc_us-gaap_GranteeStatusAxis_6bad971a-33cb-4fe7-8cbb-afa1cfc4a48d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GranteeStatusDomain_6bad971a-33cb-4fe7-8cbb-afa1cfc4a48d_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_GranteeStatusDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_GranteeStatusAxis_6bad971a-33cb-4fe7-8cbb-afa1cfc4a48d" xlink:to="loc_us-gaap_GranteeStatusDomain_6bad971a-33cb-4fe7-8cbb-afa1cfc4a48d_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GranteeStatusDomain_486f0307-69f1-4340-97fe-2978b0d819f0" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_GranteeStatusDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_GranteeStatusAxis_6bad971a-33cb-4fe7-8cbb-afa1cfc4a48d" xlink:to="loc_us-gaap_GranteeStatusDomain_486f0307-69f1-4340-97fe-2978b0d819f0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedPaymentArrangementEmployeeMember_32d624d2-cc10-4890-8b75-c7260e338b6e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedPaymentArrangementEmployeeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_GranteeStatusDomain_486f0307-69f1-4340-97fe-2978b0d819f0" xlink:to="loc_us-gaap_ShareBasedPaymentArrangementEmployeeMember_32d624d2-cc10-4890-8b75-c7260e338b6e" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.tempesttx.com/role/OrganizationandDescriptionoftheBusinessDetails" xlink:type="simple" xlink:href="tpst-20220930.xsd#OrganizationandDescriptionoftheBusinessDetails"/>
  <link:definitionLink xlink:role="http://www.tempesttx.com/role/OrganizationandDescriptionoftheBusinessDetails" xlink:type="extended" id="ie821e8132f0d48d28550731cfb4ebd70_OrganizationandDescriptionoftheBusinessDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockLineItems_183cac8c-c3c0-4f3b-a8e8-6d135357b9b8" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ClassOfStockLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueConversionOfConvertibleSecurities_7c341552-6d72-4757-b757-855ccd82d694" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StockIssuedDuringPeriodValueConversionOfConvertibleSecurities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_183cac8c-c3c0-4f3b-a8e8-6d135357b9b8" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueConversionOfConvertibleSecurities_7c341552-6d72-4757-b757-855ccd82d694" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquityReverseStockSplit_60f181b4-ffd3-40e4-99df-2cfb5045fc88" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StockholdersEquityReverseStockSplit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_183cac8c-c3c0-4f3b-a8e8-6d135357b9b8" xlink:to="loc_us-gaap_StockholdersEquityReverseStockSplit_60f181b4-ffd3-40e4-99df-2cfb5045fc88" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesIssued_9a913695-6d53-4a98-9b9e-8f18fbd9420e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CommonStockSharesIssued"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_183cac8c-c3c0-4f3b-a8e8-6d135357b9b8" xlink:to="loc_us-gaap_CommonStockSharesIssued_9a913695-6d53-4a98-9b9e-8f18fbd9420e" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_tpst_BusinessCombinationExchangeRatio_daddcecf-8a6b-477b-a13e-4409f31fdd73" xlink:href="tpst-20220930.xsd#tpst_BusinessCombinationExchangeRatio"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_183cac8c-c3c0-4f3b-a8e8-6d135357b9b8" xlink:to="loc_tpst_BusinessCombinationExchangeRatio_daddcecf-8a6b-477b-a13e-4409f31fdd73" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_tpst_StockholdersEquityNoteStockSplitExchangeRatio_82e6eb78-aa15-41fe-8c81-04027e452126" xlink:href="tpst-20220930.xsd#tpst_StockholdersEquityNoteStockSplitExchangeRatio"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_183cac8c-c3c0-4f3b-a8e8-6d135357b9b8" xlink:to="loc_tpst_StockholdersEquityNoteStockSplitExchangeRatio_82e6eb78-aa15-41fe-8c81-04027e452126" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SaleOfStockConsiderationReceivedOnTransaction_f35e548e-d894-4627-9162-05f310955d5a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_SaleOfStockConsiderationReceivedOnTransaction"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_183cac8c-c3c0-4f3b-a8e8-6d135357b9b8" xlink:to="loc_us-gaap_SaleOfStockConsiderationReceivedOnTransaction_f35e548e-d894-4627-9162-05f310955d5a" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SaleOfStockNumberOfSharesIssuedInTransaction_187fbe79-59c1-461f-8c41-3d216d106e46" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_SaleOfStockNumberOfSharesIssuedInTransaction"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_183cac8c-c3c0-4f3b-a8e8-6d135357b9b8" xlink:to="loc_us-gaap_SaleOfStockNumberOfSharesIssuedInTransaction_187fbe79-59c1-461f-8c41-3d216d106e46" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SaleOfStockPricePerShare_4aae80be-f256-430e-973d-39b966830fd8" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_SaleOfStockPricePerShare"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_183cac8c-c3c0-4f3b-a8e8-6d135357b9b8" xlink:to="loc_us-gaap_SaleOfStockPricePerShare_4aae80be-f256-430e-973d-39b966830fd8" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_tpst_SaleOfStockExercisePriceOfWarrantsOrRights_b141c8b7-1ec3-467a-a2ca-297922e421aa" xlink:href="tpst-20220930.xsd#tpst_SaleOfStockExercisePriceOfWarrantsOrRights"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_183cac8c-c3c0-4f3b-a8e8-6d135357b9b8" xlink:to="loc_tpst_SaleOfStockExercisePriceOfWarrantsOrRights_b141c8b7-1ec3-467a-a2ca-297922e421aa" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_tpst_SaleOfStockWarrantsIssued_972b427a-736a-4dc4-9a24-81d08a6192c8" xlink:href="tpst-20220930.xsd#tpst_SaleOfStockWarrantsIssued"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_183cac8c-c3c0-4f3b-a8e8-6d135357b9b8" xlink:to="loc_tpst_SaleOfStockWarrantsIssued_972b427a-736a-4dc4-9a24-81d08a6192c8" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfStockByClassTable_01465f0b-0369-422c-81bb-bed93d1a88a9" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ScheduleOfStockByClassTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ClassOfStockLineItems_183cac8c-c3c0-4f3b-a8e8-6d135357b9b8" xlink:to="loc_us-gaap_ScheduleOfStockByClassTable_01465f0b-0369-422c-81bb-bed93d1a88a9" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtConversionByUniqueDescriptionAxis_5e77bacb-0bc3-4994-8511-c5f5d54d26c9" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DebtConversionByUniqueDescriptionAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfStockByClassTable_01465f0b-0369-422c-81bb-bed93d1a88a9" xlink:to="loc_us-gaap_DebtConversionByUniqueDescriptionAxis_5e77bacb-0bc3-4994-8511-c5f5d54d26c9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtConversionNameDomain_5e77bacb-0bc3-4994-8511-c5f5d54d26c9_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DebtConversionNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtConversionByUniqueDescriptionAxis_5e77bacb-0bc3-4994-8511-c5f5d54d26c9" xlink:to="loc_us-gaap_DebtConversionNameDomain_5e77bacb-0bc3-4994-8511-c5f5d54d26c9_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtConversionNameDomain_3c46984c-da1e-487a-be92-7924bc2669f7" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DebtConversionNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtConversionByUniqueDescriptionAxis_5e77bacb-0bc3-4994-8511-c5f5d54d26c9" xlink:to="loc_us-gaap_DebtConversionNameDomain_3c46984c-da1e-487a-be92-7924bc2669f7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_tpst_ConversionOfPreferredStockIntoAShareOfCommonStockMemberMember_4c8b1d1d-1cfd-4ad0-acc6-ca6f1622ae4c" xlink:href="tpst-20220930.xsd#tpst_ConversionOfPreferredStockIntoAShareOfCommonStockMemberMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtConversionNameDomain_3c46984c-da1e-487a-be92-7924bc2669f7" xlink:to="loc_tpst_ConversionOfPreferredStockIntoAShareOfCommonStockMemberMember_4c8b1d1d-1cfd-4ad0-acc6-ca6f1622ae4c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsidiarySaleOfStockAxis_a60d3108-f3cd-4652-ac8c-d00f66a1532c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_SubsidiarySaleOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfStockByClassTable_01465f0b-0369-422c-81bb-bed93d1a88a9" xlink:to="loc_us-gaap_SubsidiarySaleOfStockAxis_a60d3108-f3cd-4652-ac8c-d00f66a1532c" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SaleOfStockNameOfTransactionDomain_a60d3108-f3cd-4652-ac8c-d00f66a1532c_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_SaleOfStockNameOfTransactionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_SubsidiarySaleOfStockAxis_a60d3108-f3cd-4652-ac8c-d00f66a1532c" xlink:to="loc_us-gaap_SaleOfStockNameOfTransactionDomain_a60d3108-f3cd-4652-ac8c-d00f66a1532c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SaleOfStockNameOfTransactionDomain_2382a459-ad65-48be-9d6f-0afb12bfa382" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_SaleOfStockNameOfTransactionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_SubsidiarySaleOfStockAxis_a60d3108-f3cd-4652-ac8c-d00f66a1532c" xlink:to="loc_us-gaap_SaleOfStockNameOfTransactionDomain_2382a459-ad65-48be-9d6f-0afb12bfa382" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_tpst_PrivateInvestmentInPublicEntityFinancingMember_964ebcae-cc05-461b-9e50-7b5434257f49" xlink:href="tpst-20220930.xsd#tpst_PrivateInvestmentInPublicEntityFinancingMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SaleOfStockNameOfTransactionDomain_2382a459-ad65-48be-9d6f-0afb12bfa382" xlink:to="loc_tpst_PrivateInvestmentInPublicEntityFinancingMember_964ebcae-cc05-461b-9e50-7b5434257f49" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_CounterpartyNameAxis_69062722-6a8b-4363-9725-d29c3a520e13" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_CounterpartyNameAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfStockByClassTable_01465f0b-0369-422c-81bb-bed93d1a88a9" xlink:to="loc_srt_CounterpartyNameAxis_69062722-6a8b-4363-9725-d29c3a520e13" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RepurchaseAgreementCounterpartyNameDomain_69062722-6a8b-4363-9725-d29c3a520e13_default" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_RepurchaseAgreementCounterpartyNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_CounterpartyNameAxis_69062722-6a8b-4363-9725-d29c3a520e13" xlink:to="loc_srt_RepurchaseAgreementCounterpartyNameDomain_69062722-6a8b-4363-9725-d29c3a520e13_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RepurchaseAgreementCounterpartyNameDomain_cc923b1c-6a07-4841-8d23-c79b8c9a2abe" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_RepurchaseAgreementCounterpartyNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_CounterpartyNameAxis_69062722-6a8b-4363-9725-d29c3a520e13" xlink:to="loc_srt_RepurchaseAgreementCounterpartyNameDomain_cc923b1c-6a07-4841-8d23-c79b8c9a2abe" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_tpst_EcoR1CapitalLLCAndVersantVentureCapitalMember_d16faab8-3fc4-4384-9d8e-2ddaf1143a84" xlink:href="tpst-20220930.xsd#tpst_EcoR1CapitalLLCAndVersantVentureCapitalMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RepurchaseAgreementCounterpartyNameDomain_cc923b1c-6a07-4841-8d23-c79b8c9a2abe" xlink:to="loc_tpst_EcoR1CapitalLLCAndVersantVentureCapitalMember_d16faab8-3fc4-4384-9d8e-2ddaf1143a84" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.tempesttx.com/role/MillendoMergerDetails" xlink:type="simple" xlink:href="tpst-20220930.xsd#MillendoMergerDetails"/>
  <link:definitionLink xlink:role="http://www.tempesttx.com/role/MillendoMergerDetails" xlink:type="extended" id="i6e96033c8e3644d0b9c85d91c71d8bb5_MillendoMergerDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionLineItems_0d540ed4-e07e-4078-bef7-b97b3e6f179e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_BusinessAcquisitionLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_4dceffa6-48ac-458c-b238-5a33a4204255" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CashAndCashEquivalentsAtCarryingValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_0d540ed4-e07e-4078-bef7-b97b3e6f179e" xlink:to="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_4dceffa6-48ac-458c-b238-5a33a4204255" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PrepaidExpenseAndOtherAssets_3170166f-16e7-4bf2-b6ad-9c6c127e7f22" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PrepaidExpenseAndOtherAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_0d540ed4-e07e-4078-bef7-b97b3e6f179e" xlink:to="loc_us-gaap_PrepaidExpenseAndOtherAssets_3170166f-16e7-4bf2-b6ad-9c6c127e7f22" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccruedLiabilitiesAndOtherLiabilities_1781f9fe-c779-4664-82d4-bc4bbbb1e71a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AccruedLiabilitiesAndOtherLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_0d540ed4-e07e-4078-bef7-b97b3e6f179e" xlink:to="loc_us-gaap_AccruedLiabilitiesAndOtherLiabilities_1781f9fe-c779-4664-82d4-bc4bbbb1e71a" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseRightOfUseAsset_87e96bc4-d9ef-4c9b-aa37-1282f710e9c5" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OperatingLeaseRightOfUseAsset"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_0d540ed4-e07e-4078-bef7-b97b3e6f179e" xlink:to="loc_us-gaap_OperatingLeaseRightOfUseAsset_87e96bc4-d9ef-4c9b-aa37-1282f710e9c5" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseLiability_c48f931b-1d60-4bd1-9520-bc0d1dc71930" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OperatingLeaseLiability"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_0d540ed4-e07e-4078-bef7-b97b3e6f179e" xlink:to="loc_us-gaap_OperatingLeaseLiability_c48f931b-1d60-4bd1-9520-bc0d1dc71930" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationAcquisitionRelatedCosts_0957b8a3-461b-420e-b506-382d126919bb" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_BusinessCombinationAcquisitionRelatedCosts"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_0d540ed4-e07e-4078-bef7-b97b3e6f179e" xlink:to="loc_us-gaap_BusinessCombinationAcquisitionRelatedCosts_0957b8a3-461b-420e-b506-382d126919bb" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_c53f75cd-6569-4c09-baf3-8fe2eac3d617" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_0d540ed4-e07e-4078-bef7-b97b3e6f179e" xlink:to="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_c53f75cd-6569-4c09-baf3-8fe2eac3d617" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAxis_337951cc-aa68-4caa-8c0f-876c391dce30" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_BusinessAcquisitionAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_c53f75cd-6569-4c09-baf3-8fe2eac3d617" xlink:to="loc_us-gaap_BusinessAcquisitionAxis_337951cc-aa68-4caa-8c0f-876c391dce30" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAcquireeDomain_337951cc-aa68-4caa-8c0f-876c391dce30_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_BusinessAcquisitionAcquireeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_BusinessAcquisitionAxis_337951cc-aa68-4caa-8c0f-876c391dce30" xlink:to="loc_us-gaap_BusinessAcquisitionAcquireeDomain_337951cc-aa68-4caa-8c0f-876c391dce30_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAcquireeDomain_f73250cb-643e-4486-9f54-4dac5c1d1d66" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_BusinessAcquisitionAcquireeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_BusinessAcquisitionAxis_337951cc-aa68-4caa-8c0f-876c391dce30" xlink:to="loc_us-gaap_BusinessAcquisitionAcquireeDomain_f73250cb-643e-4486-9f54-4dac5c1d1d66" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_tpst_PrivateTempestMemberMember_728e382e-602e-4e54-a7d6-34bf50cf4fd5" xlink:href="tpst-20220930.xsd#tpst_PrivateTempestMemberMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionAcquireeDomain_f73250cb-643e-4486-9f54-4dac5c1d1d66" xlink:to="loc_tpst_PrivateTempestMemberMember_728e382e-602e-4e54-a7d6-34bf50cf4fd5" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_56c027c7-c8bd-4ec8-a392-91788f2d45f5" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StatementEquityComponentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_c53f75cd-6569-4c09-baf3-8fe2eac3d617" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_56c027c7-c8bd-4ec8-a392-91788f2d45f5" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_56c027c7-c8bd-4ec8-a392-91788f2d45f5_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_56c027c7-c8bd-4ec8-a392-91788f2d45f5" xlink:to="loc_us-gaap_EquityComponentDomain_56c027c7-c8bd-4ec8-a392-91788f2d45f5_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_e724e209-a467-47aa-b173-62da7910cade" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_56c027c7-c8bd-4ec8-a392-91788f2d45f5" xlink:to="loc_us-gaap_EquityComponentDomain_e724e209-a467-47aa-b173-62da7910cade" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdditionalPaidInCapitalMember_f4ad9ae0-9ddb-40c6-9a6b-6293ab2f29ca" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AdditionalPaidInCapitalMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_e724e209-a467-47aa-b173-62da7910cade" xlink:to="loc_us-gaap_AdditionalPaidInCapitalMember_f4ad9ae0-9ddb-40c6-9a6b-6293ab2f29ca" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.tempesttx.com/role/FairValueMeasurementsDetails" xlink:type="simple" xlink:href="tpst-20220930.xsd#FairValueMeasurementsDetails"/>
  <link:definitionLink xlink:role="http://www.tempesttx.com/role/FairValueMeasurementsDetails" xlink:type="extended" id="ie7cbb76c1d7e4d42a3878c22987cbba7_FairValueMeasurementsDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueBalanceSheetGroupingFinancialStatementCaptionsLineItems_a8802185-c004-42ee-b73e-b3ea702e5645" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueBalanceSheetGroupingFinancialStatementCaptionsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsFairValueDisclosure_ee61f157-b629-44f0-9f5d-b1fa802d37ef" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CashAndCashEquivalentsFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueBalanceSheetGroupingFinancialStatementCaptionsLineItems_a8802185-c004-42ee-b73e-b3ea702e5645" xlink:to="loc_us-gaap_CashAndCashEquivalentsFairValueDisclosure_ee61f157-b629-44f0-9f5d-b1fa802d37ef" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsFairValueDisclosure_51a00323-87ae-41df-91fb-41a45a2df409" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AssetsFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueBalanceSheetGroupingFinancialStatementCaptionsLineItems_a8802185-c004-42ee-b73e-b3ea702e5645" xlink:to="loc_us-gaap_AssetsFairValueDisclosure_51a00323-87ae-41df-91fb-41a45a2df409" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByBalanceSheetGroupingTable_79920fb4-f5eb-447f-a2d9-f00cf3b13964" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueByBalanceSheetGroupingTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FairValueBalanceSheetGroupingFinancialStatementCaptionsLineItems_a8802185-c004-42ee-b73e-b3ea702e5645" xlink:to="loc_us-gaap_FairValueByBalanceSheetGroupingTable_79920fb4-f5eb-447f-a2d9-f00cf3b13964" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByMeasurementFrequencyAxis_52db76a3-63e5-4c96-a3b2-8f234bf6c158" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueByMeasurementFrequencyAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueByBalanceSheetGroupingTable_79920fb4-f5eb-447f-a2d9-f00cf3b13964" xlink:to="loc_us-gaap_FairValueByMeasurementFrequencyAxis_52db76a3-63e5-4c96-a3b2-8f234bf6c158" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementFrequencyDomain_52db76a3-63e5-4c96-a3b2-8f234bf6c158_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueMeasurementFrequencyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByMeasurementFrequencyAxis_52db76a3-63e5-4c96-a3b2-8f234bf6c158" xlink:to="loc_us-gaap_FairValueMeasurementFrequencyDomain_52db76a3-63e5-4c96-a3b2-8f234bf6c158_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementFrequencyDomain_75ead1b4-1697-4d0e-8b54-2c916054caa5" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueMeasurementFrequencyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByMeasurementFrequencyAxis_52db76a3-63e5-4c96-a3b2-8f234bf6c158" xlink:to="loc_us-gaap_FairValueMeasurementFrequencyDomain_75ead1b4-1697-4d0e-8b54-2c916054caa5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsRecurringMember_5edbfab5-ea32-44c1-b95c-8059e0e84d94" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueMeasurementsRecurringMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementFrequencyDomain_75ead1b4-1697-4d0e-8b54-2c916054caa5" xlink:to="loc_us-gaap_FairValueMeasurementsRecurringMember_5edbfab5-ea32-44c1-b95c-8059e0e84d94" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_063d5351-1b9c-457d-86e8-61ceb4950aba" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueByFairValueHierarchyLevelAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueByBalanceSheetGroupingTable_79920fb4-f5eb-447f-a2d9-f00cf3b13964" xlink:to="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_063d5351-1b9c-457d-86e8-61ceb4950aba" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_063d5351-1b9c-457d-86e8-61ceb4950aba_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_063d5351-1b9c-457d-86e8-61ceb4950aba" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_063d5351-1b9c-457d-86e8-61ceb4950aba_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_87b849c8-2d1d-4ca3-8935-4d93da6d8019" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_063d5351-1b9c-457d-86e8-61ceb4950aba" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_87b849c8-2d1d-4ca3-8935-4d93da6d8019" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel1Member_15a94b43-cace-41f7-a48b-61dbb1ff956e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueInputsLevel1Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_87b849c8-2d1d-4ca3-8935-4d93da6d8019" xlink:to="loc_us-gaap_FairValueInputsLevel1Member_15a94b43-cace-41f7-a48b-61dbb1ff956e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel2Member_25c12210-eea7-4535-8333-9959424be2ea" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueInputsLevel2Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_87b849c8-2d1d-4ca3-8935-4d93da6d8019" xlink:to="loc_us-gaap_FairValueInputsLevel2Member_25c12210-eea7-4535-8333-9959424be2ea" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel3Member_1793aaef-d76d-40de-8dfd-d96e60e93ff3" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueInputsLevel3Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_87b849c8-2d1d-4ca3-8935-4d93da6d8019" xlink:to="loc_us-gaap_FairValueInputsLevel3Member_1793aaef-d76d-40de-8dfd-d96e60e93ff3" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.tempesttx.com/role/BalanceSheetItemsPropertyandEquipmentNetDetails" xlink:type="simple" xlink:href="tpst-20220930.xsd#BalanceSheetItemsPropertyandEquipmentNetDetails"/>
  <link:definitionLink xlink:role="http://www.tempesttx.com/role/BalanceSheetItemsPropertyandEquipmentNetDetails" xlink:type="extended" id="i246a107cf489423b8af4ee5b12301200_BalanceSheetItemsPropertyandEquipmentNetDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentLineItems_bde15f19-c131-483d-a3d2-ba2bcf0aaee3" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PropertyPlantAndEquipmentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentGross_ede80ea1-ee82-415e-b1c1-f43ab5c1e332" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PropertyPlantAndEquipmentGross"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_bde15f19-c131-483d-a3d2-ba2bcf0aaee3" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentGross_ede80ea1-ee82-415e-b1c1-f43ab5c1e332" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment_b863445e-7812-4bfc-a76f-d8b4f400005d" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_bde15f19-c131-483d-a3d2-ba2bcf0aaee3" xlink:to="loc_us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment_b863445e-7812-4bfc-a76f-d8b4f400005d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentNet_a3eef723-7729-406f-8188-f852165079b5" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PropertyPlantAndEquipmentNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_bde15f19-c131-483d-a3d2-ba2bcf0aaee3" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentNet_a3eef723-7729-406f-8188-f852165079b5" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_0e601f74-b40b-4977-b453-4e8bb111e971" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ScheduleOfPropertyPlantAndEquipmentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_bde15f19-c131-483d-a3d2-ba2bcf0aaee3" xlink:to="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_0e601f74-b40b-4977-b453-4e8bb111e971" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_90d86cbc-4c0c-49e0-9c91-51e0dd657bf2" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PropertyPlantAndEquipmentByTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_0e601f74-b40b-4977-b453-4e8bb111e971" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_90d86cbc-4c0c-49e0-9c91-51e0dd657bf2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_90d86cbc-4c0c-49e0-9c91-51e0dd657bf2_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_90d86cbc-4c0c-49e0-9c91-51e0dd657bf2" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_90d86cbc-4c0c-49e0-9c91-51e0dd657bf2_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_db475af3-46d4-4ccf-8ec4-b3cdf5c6e270" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_90d86cbc-4c0c-49e0-9c91-51e0dd657bf2" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_db475af3-46d4-4ccf-8ec4-b3cdf5c6e270" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ComputerEquipmentMember_f0f467dc-8bb9-4bf1-9a66-b5e53ec1e1bc" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ComputerEquipmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_db475af3-46d4-4ccf-8ec4-b3cdf5c6e270" xlink:to="loc_us-gaap_ComputerEquipmentMember_f0f467dc-8bb9-4bf1-9a66-b5e53ec1e1bc" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FurnitureAndFixturesMember_7c071816-e158-4828-a568-9512d843dcdf" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FurnitureAndFixturesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_db475af3-46d4-4ccf-8ec4-b3cdf5c6e270" xlink:to="loc_us-gaap_FurnitureAndFixturesMember_7c071816-e158-4828-a568-9512d843dcdf" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquipmentMember_d42d4348-ebce-4653-8094-a6f9a7fc86cc" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_EquipmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_db475af3-46d4-4ccf-8ec4-b3cdf5c6e270" xlink:to="loc_us-gaap_EquipmentMember_d42d4348-ebce-4653-8094-a6f9a7fc86cc" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LeaseholdImprovementsMember_6869966f-9cb5-43c9-a9de-91030fcc1552" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LeaseholdImprovementsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_db475af3-46d4-4ccf-8ec4-b3cdf5c6e270" xlink:to="loc_us-gaap_LeaseholdImprovementsMember_6869966f-9cb5-43c9-a9de-91030fcc1552" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.tempesttx.com/role/CommitmentandContingenciesNarrativeDetails" xlink:type="simple" xlink:href="tpst-20220930.xsd#CommitmentandContingenciesNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.tempesttx.com/role/CommitmentandContingenciesNarrativeDetails" xlink:type="extended" id="i3bd46a8efbfa44efb50c91c4723dc3e4_CommitmentandContingenciesNarrativeDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LossContingenciesLineItems_fc67082a-4b0d-4d46-8821-7ca493e5f1a4" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LossContingenciesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseTermOfContract_8fa48012-10ef-49a0-bfb1-672eedb20bfc" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LesseeOperatingLeaseTermOfContract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingenciesLineItems_fc67082a-4b0d-4d46-8821-7ca493e5f1a4" xlink:to="loc_us-gaap_LesseeOperatingLeaseTermOfContract_8fa48012-10ef-49a0-bfb1-672eedb20bfc" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AreaOfRealEstateProperty_57aa929f-da6f-48e4-a4df-48300eb2d2b4" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AreaOfRealEstateProperty"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingenciesLineItems_fc67082a-4b0d-4d46-8821-7ca493e5f1a4" xlink:to="loc_us-gaap_AreaOfRealEstateProperty_57aa929f-da6f-48e4-a4df-48300eb2d2b4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseRemainingLeaseTerm_1519a304-d422-4129-8f2a-f8cab7857bb7" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LesseeOperatingLeaseRemainingLeaseTerm"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingenciesLineItems_fc67082a-4b0d-4d46-8821-7ca493e5f1a4" xlink:to="loc_us-gaap_LesseeOperatingLeaseRemainingLeaseTerm_1519a304-d422-4129-8f2a-f8cab7857bb7" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_tpst_NumberOfNonCancelableOperatingLeaseAgreements_32201191-04fd-4748-990c-37db770beae6" xlink:href="tpst-20220930.xsd#tpst_NumberOfNonCancelableOperatingLeaseAgreements"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingenciesLineItems_fc67082a-4b0d-4d46-8821-7ca493e5f1a4" xlink:to="loc_tpst_NumberOfNonCancelableOperatingLeaseAgreements_32201191-04fd-4748-990c-37db770beae6" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityMaximumBorrowingCapacity_705e0c5b-2eac-42b1-afd2-0f25029243c0" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LineOfCreditFacilityMaximumBorrowingCapacity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingenciesLineItems_fc67082a-4b0d-4d46-8821-7ca493e5f1a4" xlink:to="loc_us-gaap_LineOfCreditFacilityMaximumBorrowingCapacity_705e0c5b-2eac-42b1-afd2-0f25029243c0" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseRightOfUseAsset_56f41181-2d2a-4b0c-8c5d-5b1965a00c92" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OperatingLeaseRightOfUseAsset"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingenciesLineItems_fc67082a-4b0d-4d46-8821-7ca493e5f1a4" xlink:to="loc_us-gaap_OperatingLeaseRightOfUseAsset_56f41181-2d2a-4b0c-8c5d-5b1965a00c92" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseLiability_8e1aa032-ce3e-41b2-bc64-df1512bf92c0" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OperatingLeaseLiability"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingenciesLineItems_fc67082a-4b0d-4d46-8821-7ca493e5f1a4" xlink:to="loc_us-gaap_OperatingLeaseLiability_8e1aa032-ce3e-41b2-bc64-df1512bf92c0" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseExpense_1f204ddb-ea71-4eb9-9cad-1ad67c555fad" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OperatingLeaseExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingenciesLineItems_fc67082a-4b0d-4d46-8821-7ca493e5f1a4" xlink:to="loc_us-gaap_OperatingLeaseExpense_1f204ddb-ea71-4eb9-9cad-1ad67c555fad" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_tpst_LitigationExpenseAmountCoveredByInsurance_65ff8b79-aad2-49a1-a5e3-742e77efbbc2" xlink:href="tpst-20220930.xsd#tpst_LitigationExpenseAmountCoveredByInsurance"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingenciesLineItems_fc67082a-4b0d-4d46-8821-7ca493e5f1a4" xlink:to="loc_tpst_LitigationExpenseAmountCoveredByInsurance_65ff8b79-aad2-49a1-a5e3-742e77efbbc2" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InsuranceSettlementsReceivable_f2fa5cb9-7a88-4596-bb95-35003aa1f025" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_InsuranceSettlementsReceivable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingenciesLineItems_fc67082a-4b0d-4d46-8821-7ca493e5f1a4" xlink:to="loc_us-gaap_InsuranceSettlementsReceivable_f2fa5cb9-7a88-4596-bb95-35003aa1f025" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LitigationReserveCurrent_e04587e7-5068-48d7-bcb7-557fbf2b8d62" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LitigationReserveCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingenciesLineItems_fc67082a-4b0d-4d46-8821-7ca493e5f1a4" xlink:to="loc_us-gaap_LitigationReserveCurrent_e04587e7-5068-48d7-bcb7-557fbf2b8d62" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsRemainderOfFiscalYear_c2a423d3-aedf-4626-90c2-17d34921428a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsRemainderOfFiscalYear"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingenciesLineItems_fc67082a-4b0d-4d46-8821-7ca493e5f1a4" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsRemainderOfFiscalYear_c2a423d3-aedf-4626-90c2-17d34921428a" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueNextTwelveMonths_80fbed0b-a514-45d7-8e2a-25597710e945" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueNextTwelveMonths"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingenciesLineItems_fc67082a-4b0d-4d46-8821-7ca493e5f1a4" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueNextTwelveMonths_80fbed0b-a514-45d7-8e2a-25597710e945" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearTwo_b6d98df0-b59b-42de-9bb6-f4efac9ea4cd" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearTwo"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingenciesLineItems_fc67082a-4b0d-4d46-8821-7ca493e5f1a4" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearTwo_b6d98df0-b59b-42de-9bb6-f4efac9ea4cd" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearThree_c574b53f-759f-4a20-852a-99ddf64ada8a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearThree"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingenciesLineItems_fc67082a-4b0d-4d46-8821-7ca493e5f1a4" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearThree_c574b53f-759f-4a20-852a-99ddf64ada8a" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_tpst_LesseeOperatingLeaseLiabilityToBePaidDueAfterYearFour_7a64317b-34a9-4dc8-99a1-efee46d8d1bb" xlink:href="tpst-20220930.xsd#tpst_LesseeOperatingLeaseLiabilityToBePaidDueAfterYearFour"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingenciesLineItems_fc67082a-4b0d-4d46-8821-7ca493e5f1a4" xlink:to="loc_tpst_LesseeOperatingLeaseLiabilityToBePaidDueAfterYearFour_7a64317b-34a9-4dc8-99a1-efee46d8d1bb" xlink:type="arc" order="15"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LossContingenciesTable_b588f206-0172-4ab8-9e49-9bdafadfd956" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LossContingenciesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_LossContingenciesLineItems_fc67082a-4b0d-4d46-8821-7ca493e5f1a4" xlink:to="loc_us-gaap_LossContingenciesTable_b588f206-0172-4ab8-9e49-9bdafadfd956" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_StatementGeographicalAxis_dba6fd1c-10fa-44f3-b17d-371d9e4703e1" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_StatementGeographicalAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LossContingenciesTable_b588f206-0172-4ab8-9e49-9bdafadfd956" xlink:to="loc_srt_StatementGeographicalAxis_dba6fd1c-10fa-44f3-b17d-371d9e4703e1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_dba6fd1c-10fa-44f3-b17d-371d9e4703e1_default" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_StatementGeographicalAxis_dba6fd1c-10fa-44f3-b17d-371d9e4703e1" xlink:to="loc_srt_SegmentGeographicalDomain_dba6fd1c-10fa-44f3-b17d-371d9e4703e1_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_cd842333-7aa8-488a-9c13-96276244a613" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_StatementGeographicalAxis_dba6fd1c-10fa-44f3-b17d-371d9e4703e1" xlink:to="loc_srt_SegmentGeographicalDomain_cd842333-7aa8-488a-9c13-96276244a613" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_tpst_SouthSanFranciscoCaliforniaMemberMember_2aeff2d9-fc88-42f9-a90b-a2336978b81e" xlink:href="tpst-20220930.xsd#tpst_SouthSanFranciscoCaliforniaMemberMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_cd842333-7aa8-488a-9c13-96276244a613" xlink:to="loc_tpst_SouthSanFranciscoCaliforniaMemberMember_2aeff2d9-fc88-42f9-a90b-a2336978b81e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_tpst_AnnArborMichiganMemberMember_0fcdba15-bb73-4925-96d0-8f07c74fad34" xlink:href="tpst-20220930.xsd#tpst_AnnArborMichiganMemberMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_cd842333-7aa8-488a-9c13-96276244a613" xlink:to="loc_tpst_AnnArborMichiganMemberMember_0fcdba15-bb73-4925-96d0-8f07c74fad34" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_tpst_BrisbaneCaliforniaMember_fb1a0a86-d4ae-46c9-9347-655787f7722d" xlink:href="tpst-20220930.xsd#tpst_BrisbaneCaliforniaMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_cd842333-7aa8-488a-9c13-96276244a613" xlink:to="loc_tpst_BrisbaneCaliforniaMember_fb1a0a86-d4ae-46c9-9347-655787f7722d" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_tpst_SouthSanFranciscoCaliforniaMember_b51852e3-4ba0-4743-b198-80d5f9185a0d" xlink:href="tpst-20220930.xsd#tpst_SouthSanFranciscoCaliforniaMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_cd842333-7aa8-488a-9c13-96276244a613" xlink:to="loc_tpst_SouthSanFranciscoCaliforniaMember_b51852e3-4ba0-4743-b198-80d5f9185a0d" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityAxis_833f3666-a388-4f8a-a1c4-a5f3fd040898" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CreditFacilityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LossContingenciesTable_b588f206-0172-4ab8-9e49-9bdafadfd956" xlink:to="loc_us-gaap_CreditFacilityAxis_833f3666-a388-4f8a-a1c4-a5f3fd040898" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityDomain_833f3666-a388-4f8a-a1c4-a5f3fd040898_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CreditFacilityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_CreditFacilityAxis_833f3666-a388-4f8a-a1c4-a5f3fd040898" xlink:to="loc_us-gaap_CreditFacilityDomain_833f3666-a388-4f8a-a1c4-a5f3fd040898_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityDomain_f38f0d11-cfa0-449b-b146-7db312904018" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CreditFacilityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_CreditFacilityAxis_833f3666-a388-4f8a-a1c4-a5f3fd040898" xlink:to="loc_us-gaap_CreditFacilityDomain_f38f0d11-cfa0-449b-b146-7db312904018" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LetterOfCreditMember_fe052cd9-792f-44e6-b80a-1f76658d78c1" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LetterOfCreditMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CreditFacilityDomain_f38f0d11-cfa0-449b-b146-7db312904018" xlink:to="loc_us-gaap_LetterOfCreditMember_fe052cd9-792f-44e6-b80a-1f76658d78c1" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.tempesttx.com/role/CommitmentandContingenciesFutureminimumrentalpaymentsunderoperatingleaseswithnoncancelabletermsDetails" xlink:type="simple" xlink:href="tpst-20220930.xsd#CommitmentandContingenciesFutureminimumrentalpaymentsunderoperatingleaseswithnoncancelabletermsDetails"/>
  <link:definitionLink xlink:role="http://www.tempesttx.com/role/CommitmentandContingenciesFutureminimumrentalpaymentsunderoperatingleaseswithnoncancelabletermsDetails" xlink:type="extended" id="ide7bd8a1d99147d89be3122a1af08093_CommitmentandContingenciesFutureminimumrentalpaymentsunderoperatingleaseswithnoncancelabletermsDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LossContingenciesLineItems_534d2f51-462f-45cc-b277-78dbfa76e533" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LossContingenciesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsRemainderOfFiscalYear_4c9cfcc5-200c-450a-a9de-1b97996f3c05" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsRemainderOfFiscalYear"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingenciesLineItems_534d2f51-462f-45cc-b277-78dbfa76e533" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsRemainderOfFiscalYear_4c9cfcc5-200c-450a-a9de-1b97996f3c05" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueNextTwelveMonths_a2b9b4ed-1319-47e9-9022-9b2c58778779" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueNextTwelveMonths"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingenciesLineItems_534d2f51-462f-45cc-b277-78dbfa76e533" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueNextTwelveMonths_a2b9b4ed-1319-47e9-9022-9b2c58778779" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearTwo_fd10dbb0-9c78-4279-957f-80042e5cfe7c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearTwo"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingenciesLineItems_534d2f51-462f-45cc-b277-78dbfa76e533" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearTwo_fd10dbb0-9c78-4279-957f-80042e5cfe7c" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_0927b535-a55a-438a-80e5-00b196299f5a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingenciesLineItems_534d2f51-462f-45cc-b277-78dbfa76e533" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_0927b535-a55a-438a-80e5-00b196299f5a" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityUndiscountedExcessAmount_84324834-6fc6-4d65-b485-b1fda1aed555" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LesseeOperatingLeaseLiabilityUndiscountedExcessAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingenciesLineItems_534d2f51-462f-45cc-b277-78dbfa76e533" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityUndiscountedExcessAmount_84324834-6fc6-4d65-b485-b1fda1aed555" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseLiability_a59e854a-88e7-44f2-8dc1-7b2c2de0e5bd" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OperatingLeaseLiability"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingenciesLineItems_534d2f51-462f-45cc-b277-78dbfa76e533" xlink:to="loc_us-gaap_OperatingLeaseLiability_a59e854a-88e7-44f2-8dc1-7b2c2de0e5bd" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseLiabilityCurrent_802acd0b-1cb1-4f69-8d3c-7625b98dcbeb" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OperatingLeaseLiabilityCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingenciesLineItems_534d2f51-462f-45cc-b277-78dbfa76e533" xlink:to="loc_us-gaap_OperatingLeaseLiabilityCurrent_802acd0b-1cb1-4f69-8d3c-7625b98dcbeb" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseLiabilityNoncurrent_05971c68-0ca8-4473-a826-8cc7af336565" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OperatingLeaseLiabilityNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingenciesLineItems_534d2f51-462f-45cc-b277-78dbfa76e533" xlink:to="loc_us-gaap_OperatingLeaseLiabilityNoncurrent_05971c68-0ca8-4473-a826-8cc7af336565" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LossContingenciesTable_eeb6fed7-a11b-45d2-a0e1-5a7a3076a12e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LossContingenciesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_LossContingenciesLineItems_534d2f51-462f-45cc-b277-78dbfa76e533" xlink:to="loc_us-gaap_LossContingenciesTable_eeb6fed7-a11b-45d2-a0e1-5a7a3076a12e" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_StatementGeographicalAxis_ce2a9417-3de1-4dd5-916e-8c518344bd2d" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_StatementGeographicalAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LossContingenciesTable_eeb6fed7-a11b-45d2-a0e1-5a7a3076a12e" xlink:to="loc_srt_StatementGeographicalAxis_ce2a9417-3de1-4dd5-916e-8c518344bd2d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_ce2a9417-3de1-4dd5-916e-8c518344bd2d_default" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_StatementGeographicalAxis_ce2a9417-3de1-4dd5-916e-8c518344bd2d" xlink:to="loc_srt_SegmentGeographicalDomain_ce2a9417-3de1-4dd5-916e-8c518344bd2d_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_b4fc01b0-ec88-4ba0-9276-9520d5f345e4" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_StatementGeographicalAxis_ce2a9417-3de1-4dd5-916e-8c518344bd2d" xlink:to="loc_srt_SegmentGeographicalDomain_b4fc01b0-ec88-4ba0-9276-9520d5f345e4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_tpst_SouthSanFranciscoCaliforniaAndAnnArborMichiganMember_82e5f225-2620-4029-b74c-2952a9a2d538" xlink:href="tpst-20220930.xsd#tpst_SouthSanFranciscoCaliforniaAndAnnArborMichiganMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_b4fc01b0-ec88-4ba0-9276-9520d5f345e4" xlink:to="loc_tpst_SouthSanFranciscoCaliforniaAndAnnArborMichiganMember_82e5f225-2620-4029-b74c-2952a9a2d538" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.tempesttx.com/role/LoanPayableDetails" xlink:type="simple" xlink:href="tpst-20220930.xsd#LoanPayableDetails"/>
  <link:definitionLink xlink:role="http://www.tempesttx.com/role/LoanPayableDetails" xlink:type="extended" id="iac814a53e9fb42b59545e5fc47d0ecb3_LoanPayableDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentLineItems_ebc147fc-273f-4443-b86c-3671606aa4d1" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DebtInstrumentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentFaceAmount_d32a0364-a985-4aef-b68d-1e39e6e7cb81" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DebtInstrumentFaceAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_ebc147fc-273f-4443-b86c-3671606aa4d1" xlink:to="loc_us-gaap_DebtInstrumentFaceAmount_d32a0364-a985-4aef-b68d-1e39e6e7cb81" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_tpst_DebtInstrumentNumberOfTranches_f9c27866-e2cd-431c-a8d7-fa3bde18154d" xlink:href="tpst-20220930.xsd#tpst_DebtInstrumentNumberOfTranches"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_ebc147fc-273f-4443-b86c-3671606aa4d1" xlink:to="loc_tpst_DebtInstrumentNumberOfTranches_f9c27866-e2cd-431c-a8d7-fa3bde18154d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtPercentageBearingVariableInterestRate_1aa1c236-1faf-41f0-88b0-6a4b6694fd9c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LongTermDebtPercentageBearingVariableInterestRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_ebc147fc-273f-4443-b86c-3671606aa4d1" xlink:to="loc_us-gaap_LongTermDebtPercentageBearingVariableInterestRate_1aa1c236-1faf-41f0-88b0-6a4b6694fd9c" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentBasisSpreadOnVariableRate1_b5f0c00c-3558-44b3-b4cb-a5297c4585ef" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DebtInstrumentBasisSpreadOnVariableRate1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_ebc147fc-273f-4443-b86c-3671606aa4d1" xlink:to="loc_us-gaap_DebtInstrumentBasisSpreadOnVariableRate1_b5f0c00c-3558-44b3-b4cb-a5297c4585ef" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_tpst_ThersholdIndexRate_baf39dd0-5851-49b3-b94e-1a5d8f61a70f" xlink:href="tpst-20220930.xsd#tpst_ThersholdIndexRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_ebc147fc-273f-4443-b86c-3671606aa4d1" xlink:to="loc_tpst_ThersholdIndexRate_baf39dd0-5851-49b3-b94e-1a5d8f61a70f" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentPeriodicPaymentPrincipal_7cf34d9d-9e26-4e3f-a4c0-f568d1ef84f9" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DebtInstrumentPeriodicPaymentPrincipal"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_ebc147fc-273f-4443-b86c-3671606aa4d1" xlink:to="loc_us-gaap_DebtInstrumentPeriodicPaymentPrincipal_7cf34d9d-9e26-4e3f-a4c0-f568d1ef84f9" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentUnamortizedDiscountNoncurrent_168ebe5d-e216-479e-8e65-a073bb84b628" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DebtInstrumentUnamortizedDiscountNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_ebc147fc-273f-4443-b86c-3671606aa4d1" xlink:to="loc_us-gaap_DebtInstrumentUnamortizedDiscountNoncurrent_168ebe5d-e216-479e-8e65-a073bb84b628" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsOfDebtIssuanceCosts_f7ac93be-8161-4b3d-accd-c2d268ffa1a7" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PaymentsOfDebtIssuanceCosts"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_ebc147fc-273f-4443-b86c-3671606aa4d1" xlink:to="loc_us-gaap_PaymentsOfDebtIssuanceCosts_f7ac93be-8161-4b3d-accd-c2d268ffa1a7" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebt_fc18858e-74aa-48c8-9875-8bc9d24e2f99" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LongTermDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_ebc147fc-273f-4443-b86c-3671606aa4d1" xlink:to="loc_us-gaap_LongTermDebt_fc18858e-74aa-48c8-9875-8bc9d24e2f99" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NotesPayableCurrent_25521882-f6a9-4a88-b1e6-69aee01a8aeb" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NotesPayableCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_ebc147fc-273f-4443-b86c-3671606aa4d1" xlink:to="loc_us-gaap_NotesPayableCurrent_25521882-f6a9-4a88-b1e6-69aee01a8aeb" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtNoncurrent_c66a6cd9-9287-44e6-9e00-2d47601f2341" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LongTermDebtNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_ebc147fc-273f-4443-b86c-3671606aa4d1" xlink:to="loc_us-gaap_LongTermDebtNoncurrent_c66a6cd9-9287-44e6-9e00-2d47601f2341" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestExpenseDebt_970265c7-311e-4046-9e88-64c34e3b5d16" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_InterestExpenseDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_ebc147fc-273f-4443-b86c-3671606aa4d1" xlink:to="loc_us-gaap_InterestExpenseDebt_970265c7-311e-4046-9e88-64c34e3b5d16" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentTable_d1a57641-588a-4d18-8809-d275f06e3043" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DebtInstrumentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DebtInstrumentLineItems_ebc147fc-273f-4443-b86c-3671606aa4d1" xlink:to="loc_us-gaap_DebtInstrumentTable_d1a57641-588a-4d18-8809-d275f06e3043" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeAxis_998f7649-7aee-453b-b5ae-3d088bd21365" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LongtermDebtTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_d1a57641-588a-4d18-8809-d275f06e3043" xlink:to="loc_us-gaap_LongtermDebtTypeAxis_998f7649-7aee-453b-b5ae-3d088bd21365" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_998f7649-7aee-453b-b5ae-3d088bd21365_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_998f7649-7aee-453b-b5ae-3d088bd21365" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_998f7649-7aee-453b-b5ae-3d088bd21365_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_4b387db9-14d9-491a-a898-5b7adbde3c93" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_998f7649-7aee-453b-b5ae-3d088bd21365" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_4b387db9-14d9-491a-a898-5b7adbde3c93" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_tpst_TermLoanMemberMember_d2e7657f-2088-4137-b689-ab0ebef26e2a" xlink:href="tpst-20220930.xsd#tpst_TermLoanMemberMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_4b387db9-14d9-491a-a898-5b7adbde3c93" xlink:to="loc_tpst_TermLoanMemberMember_d2e7657f-2088-4137-b689-ab0ebef26e2a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_tpst_TranchATermLoanMemberMember_715244f3-23c1-4135-be25-3870e594958c" xlink:href="tpst-20220930.xsd#tpst_TranchATermLoanMemberMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_4b387db9-14d9-491a-a898-5b7adbde3c93" xlink:to="loc_tpst_TranchATermLoanMemberMember_715244f3-23c1-4135-be25-3870e594958c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_tpst_TranchBTermLoanMemberMember_81c20273-a506-431a-b832-4cdb960f33e6" xlink:href="tpst-20220930.xsd#tpst_TranchBTermLoanMemberMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_4b387db9-14d9-491a-a898-5b7adbde3c93" xlink:to="loc_tpst_TranchBTermLoanMemberMember_81c20273-a506-431a-b832-4cdb960f33e6" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_tpst_TranchCTermLoanMemberMember_d3d89558-d3b6-4c3b-baa9-de4930d149bf" xlink:href="tpst-20220930.xsd#tpst_TranchCTermLoanMemberMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_4b387db9-14d9-491a-a898-5b7adbde3c93" xlink:to="loc_tpst_TranchCTermLoanMemberMember_d3d89558-d3b6-4c3b-baa9-de4930d149bf" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.tempesttx.com/role/StockholdersEquitySummaryofConvertiblePreferredStockDetails" xlink:type="simple" xlink:href="tpst-20220930.xsd#StockholdersEquitySummaryofConvertiblePreferredStockDetails"/>
  <link:definitionLink xlink:role="http://www.tempesttx.com/role/StockholdersEquitySummaryofConvertiblePreferredStockDetails" xlink:type="extended" id="i37b352a56b8346089b8380bfa1d31d15_StockholdersEquitySummaryofConvertiblePreferredStockDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TemporaryEquityLineItems_36eef502-1ff0-4956-942e-35d3a72c4d2e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_TemporaryEquityLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TemporaryEquitySharesAuthorized_0a7446bb-38a5-456c-b722-287a51427d6b" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_TemporaryEquitySharesAuthorized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TemporaryEquityLineItems_36eef502-1ff0-4956-942e-35d3a72c4d2e" xlink:to="loc_us-gaap_TemporaryEquitySharesAuthorized_0a7446bb-38a5-456c-b722-287a51427d6b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TemporaryEquitySharesOutstanding_a6baa24d-6595-4701-9292-0d63bb53ec85" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_TemporaryEquitySharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TemporaryEquityLineItems_36eef502-1ff0-4956-942e-35d3a72c4d2e" xlink:to="loc_us-gaap_TemporaryEquitySharesOutstanding_a6baa24d-6595-4701-9292-0d63bb53ec85" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TemporaryEquityLiquidationPreferencePerShare_d734aeb9-e4ba-4f1d-9191-0ea71f97b2ea" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_TemporaryEquityLiquidationPreferencePerShare"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TemporaryEquityLineItems_36eef502-1ff0-4956-942e-35d3a72c4d2e" xlink:to="loc_us-gaap_TemporaryEquityLiquidationPreferencePerShare_d734aeb9-e4ba-4f1d-9191-0ea71f97b2ea" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TemporaryEquityLiquidationPreference_06f62c3e-f6cc-41d5-84de-47b54854a0c7" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_TemporaryEquityLiquidationPreference"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TemporaryEquityLineItems_36eef502-1ff0-4956-942e-35d3a72c4d2e" xlink:to="loc_us-gaap_TemporaryEquityLiquidationPreference_06f62c3e-f6cc-41d5-84de-47b54854a0c7" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromIssuanceOfRedeemableConvertiblePreferredStock_86ff82d0-c22a-4f70-a39e-447baeab1677" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ProceedsFromIssuanceOfRedeemableConvertiblePreferredStock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TemporaryEquityLineItems_36eef502-1ff0-4956-942e-35d3a72c4d2e" xlink:to="loc_us-gaap_ProceedsFromIssuanceOfRedeemableConvertiblePreferredStock_86ff82d0-c22a-4f70-a39e-447baeab1677" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RedeemableNoncontrollingInterestEquityPreferredCarryingAmount_0b5076dd-d642-4502-9bed-21c763bfe789" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_RedeemableNoncontrollingInterestEquityPreferredCarryingAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TemporaryEquityLineItems_36eef502-1ff0-4956-942e-35d3a72c4d2e" xlink:to="loc_us-gaap_RedeemableNoncontrollingInterestEquityPreferredCarryingAmount_0b5076dd-d642-4502-9bed-21c763bfe789" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TemporaryEquityByClassOfStockTable_c3f51d48-a410-46f2-bbe1-dd8d58828b4e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_TemporaryEquityByClassOfStockTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_TemporaryEquityLineItems_36eef502-1ff0-4956-942e-35d3a72c4d2e" xlink:to="loc_us-gaap_TemporaryEquityByClassOfStockTable_c3f51d48-a410-46f2-bbe1-dd8d58828b4e" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementClassOfStockAxis_dbf491c4-bdbf-441e-a10c-f98911d8181a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StatementClassOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_TemporaryEquityByClassOfStockTable_c3f51d48-a410-46f2-bbe1-dd8d58828b4e" xlink:to="loc_us-gaap_StatementClassOfStockAxis_dbf491c4-bdbf-441e-a10c-f98911d8181a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_dbf491c4-bdbf-441e-a10c-f98911d8181a_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementClassOfStockAxis_dbf491c4-bdbf-441e-a10c-f98911d8181a" xlink:to="loc_us-gaap_ClassOfStockDomain_dbf491c4-bdbf-441e-a10c-f98911d8181a_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_43a1db3c-ef68-4d4d-a3a0-e4f2a791617a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementClassOfStockAxis_dbf491c4-bdbf-441e-a10c-f98911d8181a" xlink:to="loc_us-gaap_ClassOfStockDomain_43a1db3c-ef68-4d4d-a3a0-e4f2a791617a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_tpst_SeriesAConvertiblePreferredStockMemberMember_8b630de6-fd3e-4da4-b2ac-7c4d64a3cf0a" xlink:href="tpst-20220930.xsd#tpst_SeriesAConvertiblePreferredStockMemberMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_43a1db3c-ef68-4d4d-a3a0-e4f2a791617a" xlink:to="loc_tpst_SeriesAConvertiblePreferredStockMemberMember_8b630de6-fd3e-4da4-b2ac-7c4d64a3cf0a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_tpst_SeriesBConvertiblePreferredStockMemberMember_e399ee3a-ab3c-40c3-bb59-9df800d271b8" xlink:href="tpst-20220930.xsd#tpst_SeriesBConvertiblePreferredStockMemberMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_43a1db3c-ef68-4d4d-a3a0-e4f2a791617a" xlink:to="loc_tpst_SeriesBConvertiblePreferredStockMemberMember_e399ee3a-ab3c-40c3-bb59-9df800d271b8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_tpst_SeriesBOneConvertiblePreferredStockMemberMember_6433e7b9-052c-4a7e-8b3f-22f05bd66b7f" xlink:href="tpst-20220930.xsd#tpst_SeriesBOneConvertiblePreferredStockMemberMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_43a1db3c-ef68-4d4d-a3a0-e4f2a791617a" xlink:to="loc_tpst_SeriesBOneConvertiblePreferredStockMemberMember_6433e7b9-052c-4a7e-8b3f-22f05bd66b7f" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.tempesttx.com/role/StockholdersEquityNarrativeDetails" xlink:type="simple" xlink:href="tpst-20220930.xsd#StockholdersEquityNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.tempesttx.com/role/StockholdersEquityNarrativeDetails" xlink:type="extended" id="i8f370d0c1e2542779d6d91fd27603ac1_StockholdersEquityNarrativeDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TemporaryEquityLineItems_5d983086-0739-4ae9-94bc-d8d819b520f2" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_TemporaryEquityLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_tpst_SaleOfStockAmountAuthorized_8bab32c6-d86f-4ce5-ba9f-1b8623c2d8c7" xlink:href="tpst-20220930.xsd#tpst_SaleOfStockAmountAuthorized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TemporaryEquityLineItems_5d983086-0739-4ae9-94bc-d8d819b520f2" xlink:to="loc_tpst_SaleOfStockAmountAuthorized_8bab32c6-d86f-4ce5-ba9f-1b8623c2d8c7" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_tpst_SaleOfStockWarrantsIssued_2fbfdd6d-3930-4ccd-8f35-468ee71ff7d3" xlink:href="tpst-20220930.xsd#tpst_SaleOfStockWarrantsIssued"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TemporaryEquityLineItems_5d983086-0739-4ae9-94bc-d8d819b520f2" xlink:to="loc_tpst_SaleOfStockWarrantsIssued_2fbfdd6d-3930-4ccd-8f35-468ee71ff7d3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_tpst_SaleOfStockExercisePriceOfWarrantsOrRights_407873fb-6956-4ddc-be41-c43f011821a5" xlink:href="tpst-20220930.xsd#tpst_SaleOfStockExercisePriceOfWarrantsOrRights"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TemporaryEquityLineItems_5d983086-0739-4ae9-94bc-d8d819b520f2" xlink:to="loc_tpst_SaleOfStockExercisePriceOfWarrantsOrRights_407873fb-6956-4ddc-be41-c43f011821a5" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_tpst_SaleOfStockWarrantCovenantsMaximumOwnershipPercentage_227f78ca-e250-4e24-992b-030773db2adf" xlink:href="tpst-20220930.xsd#tpst_SaleOfStockWarrantCovenantsMaximumOwnershipPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TemporaryEquityLineItems_5d983086-0739-4ae9-94bc-d8d819b520f2" xlink:to="loc_tpst_SaleOfStockWarrantCovenantsMaximumOwnershipPercentage_227f78ca-e250-4e24-992b-030773db2adf" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_tpst_SaleOfStockWarrantCovenantsNoticePeriod_dbfbef87-e061-4069-992e-1ff898a6ff29" xlink:href="tpst-20220930.xsd#tpst_SaleOfStockWarrantCovenantsNoticePeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TemporaryEquityLineItems_5d983086-0739-4ae9-94bc-d8d819b520f2" xlink:to="loc_tpst_SaleOfStockWarrantCovenantsNoticePeriod_dbfbef87-e061-4069-992e-1ff898a6ff29" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_tpst_SaleOfStockWarrantCovenantsMaximumOwnershipPercentageAfterIssuingNotice_064a55a8-d81a-42fe-bff2-cab34574c358" xlink:href="tpst-20220930.xsd#tpst_SaleOfStockWarrantCovenantsMaximumOwnershipPercentageAfterIssuingNotice"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TemporaryEquityLineItems_5d983086-0739-4ae9-94bc-d8d819b520f2" xlink:to="loc_tpst_SaleOfStockWarrantCovenantsMaximumOwnershipPercentageAfterIssuingNotice_064a55a8-d81a-42fe-bff2-cab34574c358" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TemporaryEquityByClassOfStockTable_332313b8-ca9f-4b5c-9912-a842fcca5c4c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_TemporaryEquityByClassOfStockTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_TemporaryEquityLineItems_5d983086-0739-4ae9-94bc-d8d819b520f2" xlink:to="loc_us-gaap_TemporaryEquityByClassOfStockTable_332313b8-ca9f-4b5c-9912-a842fcca5c4c" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_CounterpartyNameAxis_4b2cdd7b-a60f-46a9-87bb-2ab8b449f436" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_CounterpartyNameAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_TemporaryEquityByClassOfStockTable_332313b8-ca9f-4b5c-9912-a842fcca5c4c" xlink:to="loc_srt_CounterpartyNameAxis_4b2cdd7b-a60f-46a9-87bb-2ab8b449f436" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RepurchaseAgreementCounterpartyNameDomain_4b2cdd7b-a60f-46a9-87bb-2ab8b449f436_default" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_RepurchaseAgreementCounterpartyNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_CounterpartyNameAxis_4b2cdd7b-a60f-46a9-87bb-2ab8b449f436" xlink:to="loc_srt_RepurchaseAgreementCounterpartyNameDomain_4b2cdd7b-a60f-46a9-87bb-2ab8b449f436_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RepurchaseAgreementCounterpartyNameDomain_294ddfd3-d07b-4006-87f8-e9dfaf877264" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_RepurchaseAgreementCounterpartyNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_CounterpartyNameAxis_4b2cdd7b-a60f-46a9-87bb-2ab8b449f436" xlink:to="loc_srt_RepurchaseAgreementCounterpartyNameDomain_294ddfd3-d07b-4006-87f8-e9dfaf877264" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_tpst_JefferiesLLCMember_52ccdd2a-fdfc-41f5-892f-7cf82d5869d1" xlink:href="tpst-20220930.xsd#tpst_JefferiesLLCMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RepurchaseAgreementCounterpartyNameDomain_294ddfd3-d07b-4006-87f8-e9dfaf877264" xlink:to="loc_tpst_JefferiesLLCMember_52ccdd2a-fdfc-41f5-892f-7cf82d5869d1" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_tpst_EcoR1CapitalLLCAndVersantVentureCapitalMember_50482b64-ea1a-4a3c-a997-514b09c623f6" xlink:href="tpst-20220930.xsd#tpst_EcoR1CapitalLLCAndVersantVentureCapitalMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RepurchaseAgreementCounterpartyNameDomain_294ddfd3-d07b-4006-87f8-e9dfaf877264" xlink:to="loc_tpst_EcoR1CapitalLLCAndVersantVentureCapitalMember_50482b64-ea1a-4a3c-a997-514b09c623f6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsidiarySaleOfStockAxis_ea0ed456-a4e9-449c-a15c-2f1492d71155" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_SubsidiarySaleOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_TemporaryEquityByClassOfStockTable_332313b8-ca9f-4b5c-9912-a842fcca5c4c" xlink:to="loc_us-gaap_SubsidiarySaleOfStockAxis_ea0ed456-a4e9-449c-a15c-2f1492d71155" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SaleOfStockNameOfTransactionDomain_ea0ed456-a4e9-449c-a15c-2f1492d71155_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_SaleOfStockNameOfTransactionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_SubsidiarySaleOfStockAxis_ea0ed456-a4e9-449c-a15c-2f1492d71155" xlink:to="loc_us-gaap_SaleOfStockNameOfTransactionDomain_ea0ed456-a4e9-449c-a15c-2f1492d71155_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SaleOfStockNameOfTransactionDomain_231d8d91-a2cf-44eb-80a3-5dd704462d5f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_SaleOfStockNameOfTransactionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_SubsidiarySaleOfStockAxis_ea0ed456-a4e9-449c-a15c-2f1492d71155" xlink:to="loc_us-gaap_SaleOfStockNameOfTransactionDomain_231d8d91-a2cf-44eb-80a3-5dd704462d5f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_tpst_AtTheMarketProgramMember_036d59f6-7241-4658-9e5a-4b47fb1e12fa" xlink:href="tpst-20220930.xsd#tpst_AtTheMarketProgramMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SaleOfStockNameOfTransactionDomain_231d8d91-a2cf-44eb-80a3-5dd704462d5f" xlink:to="loc_tpst_AtTheMarketProgramMember_036d59f6-7241-4658-9e5a-4b47fb1e12fa" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_tpst_PrivateInvestmentInPublicEntityFinancingMember_9edf1f62-d311-4e6b-9bdc-29f8c84586c4" xlink:href="tpst-20220930.xsd#tpst_PrivateInvestmentInPublicEntityFinancingMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SaleOfStockNameOfTransactionDomain_231d8d91-a2cf-44eb-80a3-5dd704462d5f" xlink:to="loc_tpst_PrivateInvestmentInPublicEntityFinancingMember_9edf1f62-d311-4e6b-9bdc-29f8c84586c4" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.tempesttx.com/role/StockCompensationNarrativeDetails" xlink:type="simple" xlink:href="tpst-20220930.xsd#StockCompensationNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.tempesttx.com/role/StockCompensationNarrativeDetails" xlink:type="extended" id="i5ec07b4ede8c43b5afeacffb9bf82bfc_StockCompensationNarrativeDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_502a9ef5-8b6f-4c92-954a-d6ef87653b1d" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardPurchasePriceOfCommonStockPercent_e3999640-2344-4f53-9021-ed5bef2c4537" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardPurchasePriceOfCommonStockPercent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_502a9ef5-8b6f-4c92-954a-d6ef87653b1d" xlink:to="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardPurchasePriceOfCommonStockPercent_e3999640-2344-4f53-9021-ed5bef2c4537" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardAwardVestingPeriod1_fd06d9c4-0e94-452c-87d5-1e3523cb35b4" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardAwardVestingPeriod1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_502a9ef5-8b6f-4c92-954a-d6ef87653b1d" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardAwardVestingPeriod1_fd06d9c4-0e94-452c-87d5-1e3523cb35b4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardExpirationPeriod_8ca05ad1-7f64-4c0a-8385-50d91fbb6af3" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardExpirationPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_502a9ef5-8b6f-4c92-954a-d6ef87653b1d" xlink:to="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardExpirationPeriod_8ca05ad1-7f64-4c0a-8385-50d91fbb6af3" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsGrantsInPeriodGross_7555f446-d6da-48a6-90be-60aa21a25874" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsGrantsInPeriodGross"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_502a9ef5-8b6f-4c92-954a-d6ef87653b1d" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsGrantsInPeriodGross_7555f446-d6da-48a6-90be-60aa21a25874" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsGrantsInPeriodWeightedAverageExercisePrice_d1176b63-fbc4-47ae-ac5b-c2372d17e3da" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsGrantsInPeriodWeightedAverageExercisePrice"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_502a9ef5-8b6f-4c92-954a-d6ef87653b1d" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsGrantsInPeriodWeightedAverageExercisePrice_d1176b63-fbc4-47ae-ac5b-c2372d17e3da" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedStockOptions_9d6e2fee-ee37-4046-8e2a-309eb0bae296" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedStockOptions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_502a9ef5-8b6f-4c92-954a-d6ef87653b1d" xlink:to="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedStockOptions_9d6e2fee-ee37-4046-8e2a-309eb0bae296" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1_848002d9-12c0-4e26-972d-00bd00be3ba0" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_502a9ef5-8b6f-4c92-954a-d6ef87653b1d" xlink:to="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1_848002d9-12c0-4e26-972d-00bd00be3ba0" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardPercentageOfOutstandingStockMaximum_a11bac95-31c9-4f49-b4aa-2e7c1949b0f9" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardPercentageOfOutstandingStockMaximum"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_502a9ef5-8b6f-4c92-954a-d6ef87653b1d" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardPercentageOfOutstandingStockMaximum_a11bac95-31c9-4f49-b4aa-2e7c1949b0f9" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_tpst_ShareBasedCompensationArrangementByShareBasedPaymentAwardPeriodOfAutomaticIncreaseToOutstandingStockMaximum_9b21efcd-42fc-4f75-9595-5fcbbfad036e" xlink:href="tpst-20220930.xsd#tpst_ShareBasedCompensationArrangementByShareBasedPaymentAwardPeriodOfAutomaticIncreaseToOutstandingStockMaximum"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_502a9ef5-8b6f-4c92-954a-d6ef87653b1d" xlink:to="loc_tpst_ShareBasedCompensationArrangementByShareBasedPaymentAwardPeriodOfAutomaticIncreaseToOutstandingStockMaximum_9b21efcd-42fc-4f75-9595-5fcbbfad036e" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockCapitalSharesReservedForFutureIssuance_42cddffc-9fcd-41ff-9283-b3a0f73a718e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CommonStockCapitalSharesReservedForFutureIssuance"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_502a9ef5-8b6f-4c92-954a-d6ef87653b1d" xlink:to="loc_us-gaap_CommonStockCapitalSharesReservedForFutureIssuance_42cddffc-9fcd-41ff-9283-b3a0f73a718e" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardSharesIssuedInPeriod_dab251e9-2c76-46f8-8478-b8038ae638f3" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardSharesIssuedInPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_502a9ef5-8b6f-4c92-954a-d6ef87653b1d" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardSharesIssuedInPeriod_dab251e9-2c76-46f8-8478-b8038ae638f3" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_tpst_ShareBasedCompensationArrangementByShareBasedPaymentAwardMaximumNumberOfSharesAvailableForGrantPerYear_39103fb1-d33b-46b6-b4e7-f08247fa7a07" xlink:href="tpst-20220930.xsd#tpst_ShareBasedCompensationArrangementByShareBasedPaymentAwardMaximumNumberOfSharesAvailableForGrantPerYear"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_502a9ef5-8b6f-4c92-954a-d6ef87653b1d" xlink:to="loc_tpst_ShareBasedCompensationArrangementByShareBasedPaymentAwardMaximumNumberOfSharesAvailableForGrantPerYear_39103fb1-d33b-46b6-b4e7-f08247fa7a07" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfAdditionalSharesAuthorized_46e6caf8-29a6-438b-9d60-1200f4c3abbb" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfAdditionalSharesAuthorized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_502a9ef5-8b6f-4c92-954a-d6ef87653b1d" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfAdditionalSharesAuthorized_46e6caf8-29a6-438b-9d60-1200f4c3abbb" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_84af519d-40c1-4aa7-ace4-08d2edcfd62d" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_502a9ef5-8b6f-4c92-954a-d6ef87653b1d" xlink:to="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_84af519d-40c1-4aa7-ace4-08d2edcfd62d" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GranteeStatusAxis_816e6e7b-a905-4c84-babe-73d5f399972b" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_GranteeStatusAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_84af519d-40c1-4aa7-ace4-08d2edcfd62d" xlink:to="loc_us-gaap_GranteeStatusAxis_816e6e7b-a905-4c84-babe-73d5f399972b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GranteeStatusDomain_816e6e7b-a905-4c84-babe-73d5f399972b_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_GranteeStatusDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_GranteeStatusAxis_816e6e7b-a905-4c84-babe-73d5f399972b" xlink:to="loc_us-gaap_GranteeStatusDomain_816e6e7b-a905-4c84-babe-73d5f399972b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GranteeStatusDomain_ad19c413-046f-4f20-81a2-b7c4c0039486" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_GranteeStatusDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_GranteeStatusAxis_816e6e7b-a905-4c84-babe-73d5f399972b" xlink:to="loc_us-gaap_GranteeStatusDomain_ad19c413-046f-4f20-81a2-b7c4c0039486" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedPaymentArrangementEmployeeMember_9e82edff-c70c-4d7c-9e6d-7af76e881fba" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedPaymentArrangementEmployeeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_GranteeStatusDomain_ad19c413-046f-4f20-81a2-b7c4c0039486" xlink:to="loc_us-gaap_ShareBasedPaymentArrangementEmployeeMember_9e82edff-c70c-4d7c-9e6d-7af76e881fba" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanNameAxis_b1cfa2e4-af07-4cab-9b04-2878ef24731c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PlanNameAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_84af519d-40c1-4aa7-ace4-08d2edcfd62d" xlink:to="loc_us-gaap_PlanNameAxis_b1cfa2e4-af07-4cab-9b04-2878ef24731c" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanNameDomain_b1cfa2e4-af07-4cab-9b04-2878ef24731c_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_PlanNameAxis_b1cfa2e4-af07-4cab-9b04-2878ef24731c" xlink:to="loc_us-gaap_PlanNameDomain_b1cfa2e4-af07-4cab-9b04-2878ef24731c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanNameDomain_27c60ee7-9f3c-45bc-ba3e-e2694e789ee7" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_PlanNameAxis_b1cfa2e4-af07-4cab-9b04-2878ef24731c" xlink:to="loc_us-gaap_PlanNameDomain_27c60ee7-9f3c-45bc-ba3e-e2694e789ee7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_tpst_A2019EquityIncentivePlanMember_f1e8dc7e-cadb-4a67-a8ab-c110d0bda5c7" xlink:href="tpst-20220930.xsd#tpst_A2019EquityIncentivePlanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PlanNameDomain_27c60ee7-9f3c-45bc-ba3e-e2694e789ee7" xlink:to="loc_tpst_A2019EquityIncentivePlanMember_f1e8dc7e-cadb-4a67-a8ab-c110d0bda5c7" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_tpst_A2019And2017EquityIncentivePlanMember_4b00baff-a57c-4d9a-a81d-3837f34532ae" xlink:href="tpst-20220930.xsd#tpst_A2019And2017EquityIncentivePlanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PlanNameDomain_27c60ee7-9f3c-45bc-ba3e-e2694e789ee7" xlink:to="loc_tpst_A2019And2017EquityIncentivePlanMember_4b00baff-a57c-4d9a-a81d-3837f34532ae" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_tpst_EmployeeStockOwnershipPlanMember_86741dce-9c69-4d0f-a496-788b73bbba02" xlink:href="tpst-20220930.xsd#tpst_EmployeeStockOwnershipPlanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PlanNameDomain_27c60ee7-9f3c-45bc-ba3e-e2694e789ee7" xlink:to="loc_tpst_EmployeeStockOwnershipPlanMember_86741dce-9c69-4d0f-a496-788b73bbba02" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.tempesttx.com/role/StockCompensationStockBasedCompensationExpenseDetails" xlink:type="simple" xlink:href="tpst-20220930.xsd#StockCompensationStockBasedCompensationExpenseDetails"/>
  <link:definitionLink xlink:role="http://www.tempesttx.com/role/StockCompensationStockBasedCompensationExpenseDetails" xlink:type="extended" id="i3794a5b1e6e6487eb6c5ea5071962971_StockCompensationStockBasedCompensationExpenseDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_a4e79abe-a1c9-4169-bde9-7d634a90316a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AllocatedShareBasedCompensationExpense_bd965b82-c970-4a7c-81e5-a58f0cbae7ba" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AllocatedShareBasedCompensationExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_a4e79abe-a1c9-4169-bde9-7d634a90316a" xlink:to="loc_us-gaap_AllocatedShareBasedCompensationExpense_bd965b82-c970-4a7c-81e5-a58f0cbae7ba" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_4f63f1a6-03ce-4e5c-b1af-6cef6ee3dae2" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_a4e79abe-a1c9-4169-bde9-7d634a90316a" xlink:to="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_4f63f1a6-03ce-4e5c-b1af-6cef6ee3dae2" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementLocationAxis_c3a63ef9-e10b-45dc-807a-10aed7102754" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncomeStatementLocationAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_4f63f1a6-03ce-4e5c-b1af-6cef6ee3dae2" xlink:to="loc_us-gaap_IncomeStatementLocationAxis_c3a63ef9-e10b-45dc-807a-10aed7102754" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementLocationDomain_c3a63ef9-e10b-45dc-807a-10aed7102754_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncomeStatementLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_IncomeStatementLocationAxis_c3a63ef9-e10b-45dc-807a-10aed7102754" xlink:to="loc_us-gaap_IncomeStatementLocationDomain_c3a63ef9-e10b-45dc-807a-10aed7102754_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementLocationDomain_88e30e81-e559-4887-8236-c41beea5704e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncomeStatementLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_IncomeStatementLocationAxis_c3a63ef9-e10b-45dc-807a-10aed7102754" xlink:to="loc_us-gaap_IncomeStatementLocationDomain_88e30e81-e559-4887-8236-c41beea5704e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ResearchAndDevelopmentExpenseMember_ae21337c-35e6-4249-a6a5-87627567dc1c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ResearchAndDevelopmentExpenseMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_88e30e81-e559-4887-8236-c41beea5704e" xlink:to="loc_us-gaap_ResearchAndDevelopmentExpenseMember_ae21337c-35e6-4249-a6a5-87627567dc1c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GeneralAndAdministrativeExpenseMember_0aeedb2d-928f-495f-bdde-6699dc680308" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_GeneralAndAdministrativeExpenseMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_88e30e81-e559-4887-8236-c41beea5704e" xlink:to="loc_us-gaap_GeneralAndAdministrativeExpenseMember_0aeedb2d-928f-495f-bdde-6699dc680308" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.tempesttx.com/role/StockCompensationGrantDateFairValueofOptionDetails" xlink:type="simple" xlink:href="tpst-20220930.xsd#StockCompensationGrantDateFairValueofOptionDetails"/>
  <link:definitionLink xlink:role="http://www.tempesttx.com/role/StockCompensationGrantDateFairValueofOptionDetails" xlink:type="extended" id="i11a64f93713644598b61942b765c6ac9_StockCompensationGrantDateFairValueofOptionDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_be877244-30a2-48cf-b967-301401cab5f7" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardFairValueAssumptionsExpectedTerm1_dd341801-29ac-41ba-8546-065bb60bfb70" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardFairValueAssumptionsExpectedTerm1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_be877244-30a2-48cf-b967-301401cab5f7" xlink:to="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardFairValueAssumptionsExpectedTerm1_dd341801-29ac-41ba-8546-065bb60bfb70" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedVolatilityRate_138aa4e0-d92d-4bd1-8e82-64a672b3b735" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedVolatilityRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_be877244-30a2-48cf-b967-301401cab5f7" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedVolatilityRate_138aa4e0-d92d-4bd1-8e82-64a672b3b735" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsRiskFreeInterestRate_5caaf953-babd-4c96-9d5a-60e146607967" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsRiskFreeInterestRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_be877244-30a2-48cf-b967-301401cab5f7" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsRiskFreeInterestRate_5caaf953-babd-4c96-9d5a-60e146607967" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedDividendRate_883db331-bc6d-47f6-9fe8-a303a7656ec5" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedDividendRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_be877244-30a2-48cf-b967-301401cab5f7" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedDividendRate_883db331-bc6d-47f6-9fe8-a303a7656ec5" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_a4f55a31-e4a7-4638-bf4b-6b55a45d8393" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_be877244-30a2-48cf-b967-301401cab5f7" xlink:to="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_a4f55a31-e4a7-4638-bf4b-6b55a45d8393" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GranteeStatusAxis_365d76f6-855a-4126-94bf-41857484b532" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_GranteeStatusAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_a4f55a31-e4a7-4638-bf4b-6b55a45d8393" xlink:to="loc_us-gaap_GranteeStatusAxis_365d76f6-855a-4126-94bf-41857484b532" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GranteeStatusDomain_365d76f6-855a-4126-94bf-41857484b532_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_GranteeStatusDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_GranteeStatusAxis_365d76f6-855a-4126-94bf-41857484b532" xlink:to="loc_us-gaap_GranteeStatusDomain_365d76f6-855a-4126-94bf-41857484b532_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GranteeStatusDomain_909257aa-08ac-426e-b711-dc78938293f8" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_GranteeStatusDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_GranteeStatusAxis_365d76f6-855a-4126-94bf-41857484b532" xlink:to="loc_us-gaap_GranteeStatusDomain_909257aa-08ac-426e-b711-dc78938293f8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedPaymentArrangementEmployeeMember_f552fb30-c1fa-4282-9dca-f77194b1ca2d" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedPaymentArrangementEmployeeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_GranteeStatusDomain_909257aa-08ac-426e-b711-dc78938293f8" xlink:to="loc_us-gaap_ShareBasedPaymentArrangementEmployeeMember_f552fb30-c1fa-4282-9dca-f77194b1ca2d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_eb0ec940-0ee8-4b86-82d5-6002977606fa" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_a4f55a31-e4a7-4638-bf4b-6b55a45d8393" xlink:to="loc_srt_RangeAxis_eb0ec940-0ee8-4b86-82d5-6002977606fa" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_eb0ec940-0ee8-4b86-82d5-6002977606fa_default" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_eb0ec940-0ee8-4b86-82d5-6002977606fa" xlink:to="loc_srt_RangeMember_eb0ec940-0ee8-4b86-82d5-6002977606fa_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_91e8d4b1-69c4-432f-b4b6-20b4aecda195" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_eb0ec940-0ee8-4b86-82d5-6002977606fa" xlink:to="loc_srt_RangeMember_91e8d4b1-69c4-432f-b4b6-20b4aecda195" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_4ff245b5-f897-41a8-9522-14a3f0a1532f" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_91e8d4b1-69c4-432f-b4b6-20b4aecda195" xlink:to="loc_srt_MinimumMember_4ff245b5-f897-41a8-9522-14a3f0a1532f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_57a667ef-b1d3-452a-a1d3-e4f0a71d5884" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_91e8d4b1-69c4-432f-b4b6-20b4aecda195" xlink:to="loc_srt_MaximumMember_57a667ef-b1d3-452a-a1d3-e4f0a71d5884" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.tempesttx.com/role/NetLossPerShareAntidilutiveSecuritiesExcludedfromComputationofEarningsPerShareDetails" xlink:type="simple" xlink:href="tpst-20220930.xsd#NetLossPerShareAntidilutiveSecuritiesExcludedfromComputationofEarningsPerShareDetails"/>
  <link:definitionLink xlink:role="http://www.tempesttx.com/role/NetLossPerShareAntidilutiveSecuritiesExcludedfromComputationofEarningsPerShareDetails" xlink:type="extended" id="i43f61964c17248ada48c3f2f4afca9d9_NetLossPerShareAntidilutiveSecuritiesExcludedfromComputationofEarningsPerShareDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems_c97a0045-14b5-4119-91ec-14ae1b2df907" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareAmount_9728e5e6-cab5-4a6d-bfcc-656eb2e05cf8" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems_c97a0045-14b5-4119-91ec-14ae1b2df907" xlink:to="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareAmount_9728e5e6-cab5-4a6d-bfcc-656eb2e05cf8" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfAntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareTable_cdde1744-156b-4754-acaf-b0784cac67a4" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ScheduleOfAntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems_c97a0045-14b5-4119-91ec-14ae1b2df907" xlink:to="loc_us-gaap_ScheduleOfAntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareTable_cdde1744-156b-4754-acaf-b0784cac67a4" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_692d2d12-0b5f-4871-95a4-c5b344717feb" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfAntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareTable_cdde1744-156b-4754-acaf-b0784cac67a4" xlink:to="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_692d2d12-0b5f-4871-95a4-c5b344717feb" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesNameDomain_692d2d12-0b5f-4871-95a4-c5b344717feb_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AntidilutiveSecuritiesNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_692d2d12-0b5f-4871-95a4-c5b344717feb" xlink:to="loc_us-gaap_AntidilutiveSecuritiesNameDomain_692d2d12-0b5f-4871-95a4-c5b344717feb_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesNameDomain_f8979a75-ac8b-4be4-ab7f-02a7aec19506" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AntidilutiveSecuritiesNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_692d2d12-0b5f-4871-95a4-c5b344717feb" xlink:to="loc_us-gaap_AntidilutiveSecuritiesNameDomain_f8979a75-ac8b-4be4-ab7f-02a7aec19506" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeStockOptionMember_96117a2f-b7ec-4dc2-b127-0aa79a81560b" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_EmployeeStockOptionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesNameDomain_f8979a75-ac8b-4be4-ab7f-02a7aec19506" xlink:to="loc_us-gaap_EmployeeStockOptionMember_96117a2f-b7ec-4dc2-b127-0aa79a81560b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_tpst_CommonStockWarrantsMemberMember_1df8a23d-bd9e-4045-9dcb-2911020c3a59" xlink:href="tpst-20220930.xsd#tpst_CommonStockWarrantsMemberMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesNameDomain_f8979a75-ac8b-4be4-ab7f-02a7aec19506" xlink:to="loc_tpst_CommonStockWarrantsMemberMember_1df8a23d-bd9e-4045-9dcb-2911020c3a59" xlink:type="arc" order="1"/>
  </link:definitionLink>
</link:linkbase>
